Mouse anti his tag
NettetFigure 1: Sensitivity comparison of 6xHis-tag Fusion Protein by Western blot using His Antibody, mAb, Mouse from GenScript (A00186, 0.1 μg/ml) and Qiagen (34698 , 0.1 μg/ml). Figure 2: Western blot analysis of N … NettetHHHHHH. Specificity. 6X HIS Epitope Tag antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This monoclonal anti-6X …
Mouse anti his tag
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Nettet22. aug. 2014 · The high background of IHC-Fro for tumor tissues may be caused by the antibody's species. So, you can try non-mouse derived antibody, such as sheep Anti-6X His tag® antibody (ab84162) for IHC-Fro ... Nettet6X His-tag was immunoprecipitated from CHO-K1 cell lysates transfected with GFP - 6X His-tag with Mouse Anti-His-Tag-UNLB (SB Cat. No. 4603-01S). Lysates were resolved by electrophoresis, transferred to PVDF membrane, probed with Mouse Anti-His-Tag-HRP (SB Cat. No. 4603-05), and visualized using chemiluminescent detection.
NettetAnti-His 6 specifically recognizes an epitope of six consecutive histidine residues of both natural and recombinant proteins. The antibody reacts with native and denatured … Nettet24. feb. 2024 · Product Details. Monoclonal mouse Anti·His Antibodies (5xHis antibodies and 4xHis antibodies), like the RGS·His Antibody, BSA-free, Tetra·His Antibody, BSA …
NettetThis antibody was raised against a linear 8X His tag. It reacts with proteins displaying repeats of 6 Histidines (His6) or more in either internal or C and N terminal regions. It is … NettetInsect cell supernatant expressing His-tagged mouse CCL22 was resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-His Tag antibody (clone J099B12). Proteins were visualized using a goat anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection.
NettetDetection of His Tag in HEK293 Human Cell Line Transfected with His-tagged Protein by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with His-tagged protein was stained with Mouse Anti-His Tag APC-conjugated Monoclonal Antibody (Catalog # IC050A, filled histogram) or isotype control antibody (Catalog # IC002A, …
NettetImmunogen Type: Peptide. Immunogen: HIS Tag antibody was produced in mice by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide. Physical State: Liquid (sterile filtered) Concentration: 1.09 mg/mL by UV absorbance at 280 nm. Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, … monahans chamber of commerce jobsNettet1. jan. 2010 · Our anti-His tag antibody portfolio has been tested in ELISA, flow cytometry, immunofluorescence and immunoprecipitation. Clone AD1.1.10 has been successfully … monahans bayside reviewsNettetMouse Mouse anti His-Tag mAb (AE003), validaed in WB,IP. 100% Guaranteed. ABclonal provides trial size antibody samples for target detection. Replacement to … ianthellaNettetTHETM His Tag Antibody, mAb, Mouse (subtype IgG1) has very high affinity. Tests performed at GenScript show that the antibody can also recognize 4xHis- and 5xHis … monahans chemist warrnamboolNettetMonoclonal Anti-6X His tag antibody produced in mouse has been used in Co-Immunoprecipitation (Co-IP) and immunoblotting. Packaging. 100 μL. Biochem/physiol Actions. 6X His tag does not affect the structure and function of protein and can be used in antibody production. ianthe mantinghNettet13. feb. 2024 · Our 6x His Tag monoclonal, polyclonal, recombinant monoclonal and recombinant polyclonal antibodies are developed in Mouse, Rabbit, Goat, Sheep and … ian the man the mythNettetAll lanes : Anti-6X His tag® antibody [HIS.H8] (ab18184) at 1/5000 dilution Lane 1 : ARPE-19 whole cell lysate - non-transfected Lane 2 : ARPE-19 whole cell lysate - … i. ant help falling in love with you